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A novel approach to glycemic control in type 2 diabetes mellitus, partial jejunal diversion: pre-clinical to clinical pathway
  1. Martin Fried1,2,
  2. Karin Dolezalova1,2,
  3. Adam P Chambers3,
  4. Elliott J Fegelman4,
  5. Robin Scamuffa4,
  6. Michael L Schwiers4,
  7. Jason R Waggoner4,
  8. Martin Haluzik5,
  9. Randy J Seeley6
  1. 1 OB Klinika a.s, Prague, Czech Republic
  2. 2 1st Faculty of Medicine, Charles University, Prague, Czech Republic
  3. 3 GLP-1 & Obesity Pharmacology, Novo Nordisk A/S, Malov, Denmark
  4. 4 Ethicon, Inc, Cincinnati, OH, USA
  5. 5 Institute for Clinical and Experimental Medicine, Centre for Experimental Medicine, Diabetes Centre, Prague, Czech Republic
  6. 6 Department of Surgery, Internal Medicine and Nutritional Science, University of Michigan, Ann Arbor, MI, USA
  1. Correspondence to Dr Jason R Waggoner; jwaggon1{at}its.jnj.com

Abstract

Objective To explore partial jejunal diversion (PJD) via a side-to-side jejuno-jejunostomy for improved glycemic control in type 2 diabetes mellitus (T2DM). PJD is an anatomy-sparing, technically simple surgery in comparison to the predominate metabolic procedures, Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG). Positive results in a rodent model prompted a human proof-of-concept study.

Research design and methods Pre-clinically, 71 rats were studied in a model of metabolic dysfunction induced by a high-fat diet; 33 animals undergoing one of two lengths of PJD were compared with 18 undergoing sham, 10 RYGB and 10 jejuno-ileal bypass. Clinically, 15 adult subjects with treated but inadequately controlled T2DM (hemoglobin A1c (HbA1c) of 8.0%–11.0%), body mass index of 27.0–40.0 kg/m2, and C peptide ≥3 ng/mL were studied. Follow-up was at 2 weeks, and 3, 6, 9, and 12 months post-PJD.

Results Pre-clinically, positive impacts with PJD on glucose homeostasis, cholesterol, and body composition versus sham control were demonstrated. Clinically, PJD was performed successfully without serious complications. Twelve months post-surgery, the mean (SD) reduction from baseline in HbA1c was 2.3% (1.3) (p<0.01).

Conclusions PJD may provide an anatomy sparing, low-risk, intervention for poorly controlled T2DM without significant alteration of the patient’s lifestyle. The proof-of-concept study is limited by a small sample size and advanced disease, with 80% of participants on insulin and a mean time since diagnosis of over 10 years. Further study is warranted.

Trial registration number NCT02283632; Pre-results.

  • Metabolic
  • Type 2 Diabetes
  • Surgery

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

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Significance of this study

What is already known about this subject?

  • Surgical procedures such as Roux-en-Y gastric bypass and sleeve gastrectomy induce sustained improved glycemic control in patients with type 2 diabetes mellitus (T2DM) by weight loss-independent and weight loss-dependent mechanisms. One mechanistic factor involved is the introduction of nutrients more quickly and distally in the small intestine.

What are the new findings?

  • A human proof-of-concept study was successfully conducted in patients with T2DM utilizing a side-to-side jejuno-jejunostomy for partial diversion of nutrients more distally. In this small cohort of obese subjects with inadequately controlled T2DM of long duration, substantial, clinically relevant improvements in glycemic and weight control were observed.

How might these results change the focus of research or clinical practice?

  • Partial jejunal diversion may provide an anatomy sparing, low-risk, potentially reversible, metabolic procedure for patients with poorly controlled T2DM, which does not impose significant alterations in lifestyle.

Introduction

WHO reports that 422 million adults have diabetes, the majority of whom are affected by type 2 diabetes mellitus (T2DM).1 T2DM was the sixth leading cause of death in 2015.2 Bariatric metabolic surgeries (Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG)) have emerged as the most effective interventions for controlling T2DM in the obese.3 The Second Diabetes Surgery Summit (DSS-II) recently concluded that there is sufficient clinical and mechanistic evidence to support inclusion of metabolic surgery among antidiabetic interventions for T2DM and obesity.4 However, not all patients are receptive to anatomy altering surgery and lifestyle changing procedures, and more than a third of the world’s diabetic population is not obese.

Importantly, more than half the diabetic population is not under adequate glycemic control with current therapies.5 Metabolic studies indicate that introduction of nutrients more quickly and distally in the small intestine can improve glucose homeostasis. A recent study6 indicates that bariatric surgery can result in complete or partial T2DM remission in a majority of patients for at least 5 years and provides the impetus to develop less invasive metabolic procedures.7 The jejuno-ileal bypass (JIB),8 9 the duodenal-jejunal bypass (DJB),7 DJB with SG, the single-anastomosis duodenoileal bypass with SG (SADI-S),10 and the SG plus side-to-side jejuno-ileal anastomosis11 procedures have shown improvements in glucose homeostasis but have had limited adoption due to a combination of surgical complications and negative impact on lifestyle.

A rodent model with a side-to-side jejuno-jejunostomy is reported here which indicates that a partial diversion of stomach contents in the small intestine improves glycemic control with less weight loss than current bariatric metabolic surgeries. By utilizing a side-to-side jejuno-jejunostomy, complications such as severe diarrhea seen with the JIB procedure may be avoided. Partial jejunal diversion (PJD) entails creating a side-to-side anastomosis that allows a portion of nutrients to bypass the intact loop of bowel while the remaining portion of nutrients follows the common path of intestinal transit. This procedure intentionally eliminates the blind limb associated with JIB. Given the demonstrated improvements in glucose tolerance in a pre-clinical model, a proof-of-concept study was undertaken in patients with poorly controlled T2DM with a body mass index (BMI) of 27–40 kg/m2.

Research design and methods

Rat model of diet-induced obesity and metabolic impairment

Male Long-Evans rats (n=71) (250–300 g; Harlan Laboratories, Indianapolis, Indiana, USA) were individually housed and maintained on a 12/12 hour light/dark cycle (lights off at 18:00 hours) at 25°C and 50%–60% humidity. All procedures for animal use were approved by the University of Cincinnati Institutional Animal Care and Use Committee. Before surgery, rats were given ad libitum access to water and a high-fat diet (4.54 kcal/g; 41% fat; Research Diets, New Brunswick, New Jersey, USA) previously documented to produce metabolic impairments.12 After 8 weeks on the high-fat diet, rats were assigned to one of five surgical groups (sham, JIB, 30 cm PJD, 42 cm PJD or RYGB) that were matched for fat tissue mass. At 3 days pre-operatively, the high-fat diet was temporarily replaced with Ensure Plus liquid diet (1.41 kcal/g; 29% fat) (Abbott Nutrition, Columbus, Ohio, USA) for 7 days after which the rats were returned to the same high-fat diet as before. Subcutaneous injections of Metacam (0.25 mg/100 g body weight (BW) once daily for 4 days), gentamicin (0.8 mg/100 g BW on the day of surgery), Buprenex (0.3 mL 2X per day for 5 days), and warm saline (10 and 5 mL 2X per day for days 0–3 and 4–5, respectively) were given to all post-operative rats. On the day of surgery, rats were anesthetized under isoflurane and pre-treated with analgesics and gentamicin as above.

Rodent models

For the sham operation, a laparotomy was performed to gain access to the abdominal cavity, and a section of jejunum was isolated and cut 30 cm beyond the ligament of Treitz. The two halves were then anastomosed end-to-end using a running stitch with 7–0 Vicryl absorbable suture (Ethicon, Somerville, New Jersey, USA). The laparotomy was closed in layers. For the 30 cm PJD, an anti-mesenteric incision was made 30 cm beyond the ligament of Treitz and another 30 cm from the ileocecal junction. A running stitch with 8–0 suture was used to join the two sections of bowel with reinforcements placed at the mid-point of each semi-circular section with an interrupted stitch. The resulting loop was roughly 30 cm long. The 42 cm PJD was identical except that the distal incision was made 18 cm from the ileocecal junction. For JIB an anti-mesenteric incision was made 30 cm from the ileocecal junction. The duodenum was transected 10 cm beyond the pylorus and the distal jejunal remnant was closed. The two open sections of bowel were then connected using a running stitch with reinforcements at each mid-point. To perform RYGB, the jejunum was transected 30 cm beyond the ligament of Treitz. An anti-mesenteric incision was made 10 cm distal to the transected bowel and connected to the afferent limb with a running absorbable suture. The stomach was isolated and the fundus was excised by making a vertical cut along the edge of the corpus with an ETS Articulating Linear Cutter (Ethicon, Cincinnati, Ohio, USA). A second staple line was placed across the waist of the stomach, creating a gastric pouch that was 10% the size of the original stomach. The distal remnant was returned to the peritoneal cavity and an incision was made on the gastric pouch that spared the vascular architecture. The efferent limb of the transected jejunum was then connected to the gastric pouch with a running 8–0 Prolene non-absorbable suture (Ethicon, Somerville). All incisions used to create anastomoses were 8 mm long and in all cases the abdominal wall was closed in layers using a running stitch and a running subcuticular stitch.

Rodent food intake and fat absorption

A pilot study was performed in a separate cohort of rats (n=22/71) from those used in the meal tolerance test to assess the effect of PJD on food intake and fat absorption. Food intake was monitored weekly after surgery in sham (n=8), 30 cm PJD (n=7), or 42 cm PJD (n=7) treatment groups and fat absorption was assessed after 4 weeks using the Behenate method, as described previously.13 Briefly, the high-fat diet was removed and rats were temporarily placed on a diet containing 5% sucrose polybehenate (behenic acid). After 24 hours of acclimation to the diet, cages were changed and fecal pellets were collected after another 24 hours. Fecal samples of about 10 mg were collected and fecal lipid content was assayed by gas chromatography of fatty acid methyl esters. Fat absorption was calculated from the ratio of behenic acid to other fatty acids in the diet and feces.13

Rodent mixed-meal tolerance test

Blood glucose, glucagon-like peptide-1 (GLP-1), and insulin response to a mixed-meal tolerance test (MMTT) were assessed in rats that underwent sham (n=10), 30 cm PJD (n=8), 42 cm PJD (n=11), JIB (n=10), or RYGB (n=9) procedures. Approximately 5 weeks after surgery, 5-hour fasted rats were gavaged with 2.8 mL Ensure Plus Liquid diet. Blood samples (5 µL) for glucose were collected at 0, 15, 30, 45, 60, 120 min post-gavage, and analyzed by a hand-held glucometer to quantify glucose concentrations. An additional 180 µL aliquot of blood was collected into tubes containing 20 L antiproteolytic cocktail (4.65 g EDTA, 92 mg aprotinin, 40 000 U heparin in 50 mL saline) at baseline and 15 min after gavage to assess post-prandial plasma GLP-1 and insulin release. The 15 min time point was chosen to coincide with peak plasma insulin and GLP-1 levels in sham and RYGB rats observed previously by our group.14 We do not know if this time point coincides with peak levels in PJD rats. Samples were centrifuged (20 min at 9000 rpm) and plasma was collected and immediately frozen until analysis. GLP-1 was analyzed by an electrochemiluminescence assay (Meso Scale Discovery, Gaithersburg, Maryland, USA) and plasma insulin was measured using a rat insulin ELISA (Crystal Chem, Downers Grove, Illinois, USA).

Rodent body composition

Lean and fat tissue mass were assessed using an EchoMRI analyzer (Houston, Texas, USA) 1 week prior to surgery and again after 4 weeks in sham (n=10), 30 cm PJD (n=8), 42 cm PJD (n=10), JIB (n=10), and RYGB (n=9) rats. A rat in the 42 cm PJD group died prior to the final body weight measurement on day 56 and was excluded from body composition and liver triglyceride (TG) analyses.

Rodent liver TG

On day 56, the same rats used in the MMTT were sacrificed using CO2 asphyxiation. Livers were flash frozen in isopentane, and lipid from 50 mg of tissue was extracted in 2:1 chloroform/methanol. TG content was measured via colorimetric assays using Infinity Reagents (Thermo Fisher Scientific, Waltham, Massachusetts, USA).

Clinical study design, recruitment, and enrollment of participants

Fifteen patients underwent PJD between November 2014 and May 2015 at the OB Clinic, Prague, Czech Republic. The study was approved by the Ethics Committee of the OB Clinic and informed consent was obtained from all study patients. The study was conducted in accordance with the Declaration of Helsinki and was registered at ClinicalTrials.gov supported by the United States National Institutes of Health as NCT02283632.

Key inclusion criteria included hemoglobin A1c (HbA1c) of 8.0%–11.0%, BMI 27.0–39.9 kg/m2, age 20–60 years, C peptide ≥3 ng/mL, and presence of at least one other cardiovascular risk factor: hypertension (systolic blood pressure (BP) ≥140 mm Hg and/or diastolic BP ≥90 mm Hg) or anti-hypertensive medication use; dyslipidemia (high-density lipoprotein cholesterol (HDL-C) <40 mg/mL (men) or <50 mg/mL (women); low-density lipoprotein cholesterol (LDL-C) >100 mg/mL; or TG ≥150 mg/dL) or dyslipidemia medication use.

Key exclusion criteria included any previous major gastrointestinal (GI) resection, type 1 diabetes mellitus, latent autoimmune diabetes of adulthood, screening laboratory test abnormalities (alanine aminotransferase (ALT) and/or aspartate aminotransferase (AST) levels ≥4 times upper limit of normal (ULN); blood creatinine level ≥1.5 times ULN; or blood urea nitrogen (BUN) level ≥1.5 times ULN), or prescription or over-the-counter medications or supplements with a primary indication known to cause or assist in weight reduction.

Procedures

All patients had a physical examination conducted, and fasting blood laboratory samples, medical history, concomitant medication usage, vital signs and anthropometric data collected prior to PJD. Follow-up visits were conducted at 2 weeks, 3, 6, 9, and 12 months after the procedure. Per local medical practice, patients were hospitalized and discharged only after normal bowel movement. For each visit, vital signs, anthropometric data, fasting blood laboratory data, and concomitant medication usage were recorded. Oral glucose tolerance testing (OGTT) was performed at the baseline visit and at 3 and 12 months post-procedure. A CT enterography (CTE) scan was conducted to assess anastomotic patency approximately 9–12 months after PJD. The scans were read by an independent radiologist to subjectively assess percent of nutrient diversion.

Surgical technique

Patients underwent general anesthesia, and optimal laparoscopic access and pneumoperitoneum were established. Four trocars (two 5 mm and two 10/12 mm) were placed. One 10/12 mm was introduced in the mid-hypogastric region and the second 10/12 mm mid-distance between the right iliac spine and umbilicus. One 5 mm trocar was placed on the patient’s left, opposite to the 10/12 mm in the left iliac region and the second 5 mm trocar was placed in the patient’s left flank on the umbilical level. The omentum was retracted cephalad while simultaneously exposing the ligament of Treitz. The jejunum was measured 100 cm from the ligament of Treitz and marked with a suture. A length of 250 cm of ileum was measured from the ileocecal junction. At the site, the jejunal limb was approximated and aligned with the ileum in an isoperistaltic manner. The anti-mesenteric borders of the jejunal and ileal limbs were opened using an advanced energy bipolar device. Two enterotomies were created and a 60 mm long linear stapler was used to create a side-to-side anastomosis. The lumen was inspected for bleeding and the enterotomy was hand sutured closed in two layers. The mesenteric defect along the anastomosis was closed by running suture. Finally, the abdomen was deflated and the port sites were closed.

Outcomes

All subjects were assessed for HbA1c at 2 weeks, and 3, 6, 9, and 12 months post-procedure and change from baseline in HbA1c was calculated. Other pre-specified outcome measurements included safety, change from baseline in glucose homeostasis (blood glucose area under curve (AUC) values at each visit were calculated using the linear trapezoidal rule based on the fasting value prior to the glucose tolerance test and the subsequent values collected at 30 min, and 1, 2, 3, 4, and 5 hours after the glucose tolerance test), whole-body insulin sensitivity (assessed from the OGTT data using the Matsuda Index15), β-cell function (assessed using the ratio of AUC C peptide/AUC glucose calculated from the OGTT data16), lipid levels, liver and renal function, and usage of anti-hyperglycemic, anti-hypertensive, and dyslipidemia medications.

Statistical analysis

Rodent data were analyzed via one-way independent analysis of variance (ANOVA) or two-way ANOVA with time as a repeated measure, followed by Bonferroni’s multiple comparisons tests, or, unpaired t-tests where indicated. Data are expressed as mean±SEM.

For statistical analyses of all clinical data, SAS, V.9.3 (Cary, North Carolina, USA) was used. Summary statistics including n, mean, SD, median, minimum, and maximum were provided for the observed values at the given time point as well as the change from baseline at that time point. Additionally, 95% CIs were estimated for the change from baseline and the mean change from baseline was tested using the one-sample t-test or the Wilcoxon signed-rank test as appropriate. A significance level of 0.05 was used to determine statistical significance. For all analyses, baseline was defined as the last measurement obtained prior to PJD being performed.

Results

Pre-clinical results

During the first 2 weeks post-operatively, food intake was significantly reduced in rats undergoing 30 cm PJD (731±40 g, n=8) and 42 cm PJD (650±77 g, n=7) relative to sham-operated rats (1054±73 g, n=8), after which differences in weekly food consumption were non-significant among groups (data not shown). Fat absorption, expressed as a percent of dietary content absorbed, was slightly reduced in rats that underwent 42 cm PJD (75%±3.3%, n=7) relative to sham-operated rats (83%±1.6%, n=8; p=0.03), whereas differences in fat absorption between sham and 30 cm PJD (77%±2.8%, n=8) rats were non-significant.

In a second cohort of rats, blood glucose, plasma GLP-1, and plasma insulin levels were assessed during a MMTT performed approximately 5 weeks after surgery. Figure 1B shows that both 30 cm (at 15 min) and 42 cm (at 120 min) versions of PJD resulted in significantly lower blood glucose excursions than sham during the MMTT. As expected, plasma GLP-1 levels (Figure 1C) were elevated after RYGB and JIB 15 min post-MMTT relative to sham-operated rats, and to a greater extent than after PJD. However, this may have been different at later time points. Interestingly, both fasting and post-challenge plasma insulin levels (Figure 1D) were significantly lower after 30 cm PJD than sham, implying greater insulin sensitivity as body weight, body composition, and liver TG levels were similar among these groups (Figure 1A,E–G). RYGB and JIB resulted in greater blood glucose lowering than PJD but also evoked significantly more weight loss.

Figure 1

Body weight and oral glucose tolerance in diet-induced obese rats at 5 weeks post-surgery. Diet-induced obese rats underwent a sham (n=10) operation, 30 cm partial jejunal diversion (PJD) (n=8), 42 cm PJD (n=10), jejuno-ileal bypass (JIB) (n=10), or Roux-en-Y gastric bypass (RYGB) (n=9) surgery. (A) Body weight (g); (B) blood glucose in response to a 2-hour mixed meal tolerance test (MMTT, n=7–11 per group); (C) plasma GLP-1 (pg/mL, 8–10 per group); (D) plasma insulin (ng/mL, n=8–10); (E) 4-week lean mass (g, n=8–10 per group); F) 4-week fat mass (g, n=8–10 per group); (G) liver triglycerides (mg/dL). All data presented as mean±SE. Data were analyzed via one-way independent measures analysis of variance (ANOVA) or two-way ANOVA with time as a repeated measure, followed by Bonferroni multiple comparisons test where appropriate. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, unpaired t-tests were performed where indicated.

Clinical results

Patient characteristics

Baseline characteristics are shown in table 1 for the 15 subjects undergoing PJD.

Table 1

Patient baseline characteristics

Procedure

PJD was successfully completed in all 15 patients. The mean procedure duration was 104.5 min (range: 70–140 min). The average length of hospital stay was 3.3 nights (range: 2–7 nights). All patients were discharged uneventfully and returned to normal diet immediately. There were no readmissions after discharge. CTE scans showed that the anastomosis was patent at 9–12 months after the procedure. The mean oral jejunum to oral ileum flow was estimated to be 57%–43%, respectively.

HbA1c and glucose homeostasis

Mean HbA1c (figure 2A and table 2) and fasting blood glucose (table 2) were significantly lower by 2 weeks after PJD, and continued to decrease over 12 months. Eight of 15 patients (53.3%) had a >2% absolute reduction and 11 of 15 patients (73.3%) had a >1.5% absolute reduction in HbA1c 12 months post-PJD, and 7 patients (46.7%) had achieved a HbA1c <7.0%. Compared with baseline at 12 months post-PJD, OGTT showed a significant decrease in blood glucose AUC, a significant increase in β-cell function, a significant increase in GLP-1 AUC, and an increase in whole-body insulin sensitivity.

Figure 2

Key clinical efficacy outcomes for (A) hemoglobin A1c (HbA1c), (B) weight change. Data points are mean change from baseline absolute HbA1c (A) or mean change from baseline absolute body weight (B) and error bars represent SEM for n=15 patients. Data below the charts are absolute mean and median HbA1c (A) or mean and median % body weight reduction (*p<0.05) compared with baseline values.

Table 2

Study metabolic variables

Weight change

Significant weight loss was first observed 2 weeks after PJD and continued over 12 months (figure 2B and table 2). Total weight loss varied from 2 to 21 kg with a mean of 10.8 kg (corresponding to a 10.3% reduction from baseline weight). Ten of 15 patients (66.7%) lost ≥10% of their body weight and 13 of 15 patients (86.7%) lost ≥5%. Concurrent reductions were also observed in BMI and waist circumference.

Other cardiometabolic outcomes

Mean fasting insulin decreased significantly in conjunction with mean LDL-C and TG at 12 months post-procedure and there were no significant changes in mean HDL-C (table 2). Mean systolic BP decreased significantly at 12 months post-procedure, whereas diastolic BP remained essentially unchanged over the 12-month post-procedure period (table 2). All liver function values (except for serum albumin) decreased significantly at 12 months post-procedure (table 2). For renal function, creatinine remained similar to baseline values, but BUN increased significantly at 12 months post-procedure (table 2). Vitamin B-12 levels decreased significantly at 12 months post-procedure (table 2).

Pre-PJD, 14 patients were taking at least one anti-hyperglycemic agent (AHA) of whom 12 were receiving insulin; one patient had stopped all anti-hyperglycemic medication before entry into the study. Twelve months after PJD, 13 patients still required at least one AHA of whom 8 were receiving insulin, although 4 patients decreased the number of AHAs they received. Among the 12 patients requiring insulin at the start of the study, 4 stopped insulin treatment (baseline dose ranged from 30 to 80 units); 4 continued on insulin with an average reduction in their approximate daily dose of 54% (mean reduction of 55 units with range from −30 to −84 units); and another 4 maintained the same approximate daily dose of insulin (unit change of no more than 10 units). There were no major changes in number of patients receiving anti-hypertensive and dyslipidemia medications at study end (table 3).

Table 3

Concomitant medication usage

Clinical adverse events

All adverse events (AEs) regardless of type, severity, or relationship to the procedure were recorded for the duration of the study. There were 36 procedure-related AEs recorded in 13 subjects (86.7%) and one was considered severe, 11 were considered moderate, and 24 were considered mild. These procedure-related AEs were also classified for clinical relevance per the Clavien-Dindo surgical complications scale.17 There were 32 AEs at the lowest grade (grade I), 4 were grade II, and none reached grades III–V. No event resulted in surgical intervention. Fifteen events required no intervention; 2 resolved with diet; 19 resolved with medication including one episode of lower extremity edema (considered severe) that resolved during a 2-day hospitalization.

Among the 13 subjects experiencing a procedure-related AE, the most common AEs were diarrhea in 7 subjects (46.7%), flatulence in 4 subjects (26.7%), and pain in 5 subjects (33.3%). Diarrhea dissipated between 2 and 10 days after onset and was considered mild to moderate. One episode of dehydration, not considered procedure-related, prompted hospitalization 3 months after the procedure, but was not associated with diarrhea and resolved without sequelae.

Conclusions

Based on the improvements in oral glucose tolerance in a rodent model of diet-induced obesity and metabolic impairment, PJD demonstrated potential as a surgical approach to treat T2DM. These data show that PJD is mildly or non-malabsorptive depending on distance of the intestinal loop along the GI tract. Overall, differences among 30 and 42 cm versions of PJD in the pre-clinical studies were subtle. There was a trend for the 42 cm PJD version of treated rats to weigh less and fat absorption was reduced by approximately 10% in this group relative to sham-treated rats, whereas fat absorption was unaffected in 30 cm PJD rats. In comparison, we previously showed that RYGB surgery reduces fat absorption by roughly 30% in this model.18 Taken together, these data show that PJD has the potential to benefit oral glucose handling without causing pronounced or drastic significant malabsorption or changes in body weight in a pre-clinical rat model. We do not yet know if, or, how these findings will translate to PJD in humans.

Clinically, PJD was executed as anticipated without incident, and was generally well-tolerated by the patients. Given historical experience with serious complications after JIB,8 9 PJD was designed with a partial diversion to avert the complications associated with a blind loop, full bypass. AEs were scrupulously recorded and rigorously addressed in this proof-of-concept study, resulting in an observed procedure-related AE rate of 86.7%. However, classification of these events by their clinical relevance using the Clavien-Dindo scale indicated a favorable safety profile with all events at the grade I or II level and none at grades III–V. A recent evaluation19 of patients with diabetes undergoing an RYGB using a composite of complications (considered grade III or higher on the Clavien-Dindo scale) reported a 3.4% complication rate. This work observed none of these complications, although it is recognized that this initial cohort is too small to observe events with a low incidence, thus warranting further observation in a larger population.

Even in this small cohort of predominantly obese subjects with advanced T2DM, substantial, clinically relevant improvements in glycemic and weight control were observed with attendant reduction in AHA usage. Based on previous work by Gummesson et al,20 one would expect for every 1 kg of mean weight loss there is a corresponding decrease of 0.1 percentage unit in HbA1c. Therefore, in this work with a mean weight loss of 10.8 kg, we would expect an approximate 1.1% unit decrease in HbA1c. However, a 2.3% unit decrease in HbA1c was observed suggesting some weight-independent effect on HbA1c.

Concurrent with the clinical work described here, Melissas et al 21 published outcomes of a similar procedure in a cohort of six patients with T2DM that underwent a simple side-to-side jejuno-ileal anastomosis. Their preliminary results showed prolonged T2DM remission in three patients and partial remission in the other three patients post-operatively. Follow-up ranged from 6 to 42 months. Several differences between the study populations point to patients in the study by Melissas et al as having greater β-cell function and being earlier in T2DM progression: 17% vs 80% of patients on insulin prior to the procedure, average time since T2DM diagnosis of just over 5 years versus over 10 years, and median baseline HbA1c level of 7.6% (n=6; range: 6.7%–8.4%) vs 9.4% (n=15; range: 8.1%–10.8%). Patients in both studies showed clinically relevant mean total weight loss of at least 10% post-procedure.

This proof-of-concept study is limited by a small study population with advanced disease, 80% of whom were insulin dependent and an average time since T2DM diagnosis of over 10 years, both of which are factors which have been shown to blunt the effect of bariatric surgery on T2DM.22 Several other limitations include lack of metabolic biomarkers, length of follow-up after the procedure, and no pre-determined standardized treatment algorithm for adjusting AHAs. Future studies of PJD should include longer follow-up to determine durability of effect and further characterization of mechanisms of action. Metabolic surgery involving resection such as RYGB and SG are recognized among the most potent interventions for T2DM available, but are not appropriate for overweight or normal weight patients and not routinely accepted by patients with obesity or referring physicians.

In conclusion, the results from these studies suggest that PJD may provide an anatomy sparing, low-risk, potentially reversible, metabolic procedure for poorly controlled T2DM, which does not impose significant alteration of a patient’s lifestyle and may reduce the need for multiple medications. Further studies to confirm efficacy, safety, and durability of effect in T2DM are warranted.

Acknowledgments

The authors wish to acknowledge the early study design input and feedback from Dr Muffazal Lakdawala (Centre for Obesity and Digestive Surgery, Mumbai, India), Dr Dave D’Alessio (Division of Endocrinology, Metabolism and Nutrition, Duke University, Durham, North Carolina, USA) and Dr Nestor de la Cruz-Munoz (Division of Laparoendoscopic and Bariatric Surgery, Florida International University, Miami, Florida, USA), and scientific input from Dr Kirk Ways and Dr David Polidori of Janssen R&D USA.

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View Abstract

Footnotes

  • Contributors MF was involved in the clinical study design, execution, data collection and interpretation, and manuscript review and final approval. KD was involved in clinical study execution, data collection and interpretation, and manuscript review and final approval. APC was involved in the pre-clinical study design, execution, data collection and interpretation of the pre-clinical work, and manuscript preparation and final approval. EJF was involved in the clinical study design, data interpretation, and manuscript review and final approval. RS was involved in data interpretation, manuscript preparation and final approval. MLS was involved in the clinical study design, statistical analysis, data interpretation, manuscript review and final approval. JRW was involved in the clinical study design, data interpretation, manuscript preparation and final approval. MH was involved in the data interpretation, manuscript review and final approval. RJS was involved in the pre-clinical study design, data collection and interpretation of the pre-clinical work, clinical study design, data interpretation, manuscript review and final approval. All authors have reviewed and approved the completed manuscript.

  • Funding Funding for this study has been provided by Ethicon, Inc and by RVO VFN64165 from MHCR to MH.

  • Competing interests MF, KD, APC, and MH have nothing to declare. RJS currently receives funding from Ethicon, Inc. EJF, RS, MLS, and JRW are employed by Ethicon, Inc.

  • Patient consent Informed consent was previously obtained from all study patients.

  • Ethics approval Ethics Committee of the OB Clinic, Prague, Czech Republic.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data sharing statement This manuscript includes all the data available.

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