Primer sequences for real-time RT-PCR
| Symbol | Forward primer | Reverse primer |
|---|---|---|
| Caspase-1 | GAGCTGATGTTGACCTCAGAG | CTGTCAGAAGTCTTGTGCTCTG |
| TNFα | GACCCTCACACTCAGATCATCTTCT | TGCTACGACGTGGGCTACG |
| IL-1β | CCCTGCAGCTGGAGAGTGTGG | TGTGCTCTGCTTGAGAGGTGCT |
| IL-6 | TAGTCCTTCCTACCCCAATTTCC | TTGGTCCTTAGCCACTCCTTC |
| IL-18 | AAGAACAAGATCATTTCCTTTGAGGA | GGAACACGTTTCTGAAAGAATATGA |
| ASC | CTCTGTATGGCAATGTGCTGAC | GAACAAGTTCTTGCAGGTCAG |
| GAPDH | ATGATTCTACCCACGGCAAG | CTGGAAGATGGTGATGGGTT |
ASC, apoptosis-associated speck-like protein; IL, interleukin; TNFα, tumor necrosis factor α.