miR-466 is putative negative regulator of Coxsackie virus and Adenovirus Receptor

FEBS Lett. 2015 Jan 16;589(2):246-54. doi: 10.1016/j.febslet.2014.12.006. Epub 2014 Dec 10.

Abstract

This study aimed at elucidating how Coxsackie B virus (CVB) perturbs the host's microRNA (miRNA) regulatory pathways that lead to antiviral events. The results of miRNA profiling in rat pancreatic cells infection models revealed that rat rno-miR-466d was up-regulated in CVB infection. Furthermore, in silico studies showed that Coxsackie virus and Adenovirus Receptor (CAR), a cellular receptor, was one of the rno-miR-466d targets involved in viral entry. Subsequent experiments also proved that both the rno-miR-466d and the human hsa-miR-466, which are orthologs of the miR-467 gene family, could effectively down-regulate the levels of rat and human CAR protein expression, respectively.

Keywords: Coxsackie B virus; Coxsackie virus and Adenovirus Receptor; microRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Coxsackie and Adenovirus Receptor-Like Membrane Protein / chemistry
  • Coxsackie and Adenovirus Receptor-Like Membrane Protein / genetics*
  • Enterovirus B, Human / metabolism*
  • Gene Expression Regulation
  • Humans
  • MicroRNAs / genetics*
  • Molecular Sequence Data
  • Rats

Substances

  • Clmp protein, rat
  • Coxsackie and Adenovirus Receptor-Like Membrane Protein
  • MIRN466 microRNA, human
  • MIRN466 microRNA, rat
  • MicroRNAs