Article Text
Abstract
Objective We assessed the therapeutic effects of photobiomodulation (PBM) and adipose-derived stem cell (ADS) treatments individually and together on the maturation step of repairing of a delayed healing wound model in rats with type 1 diabetes mellitus (DM1).
Research design and methods We randomly assigned 24 rats with DM1 to four groups (n=6 per group). Group 1 was the control (placebo) group. In group 2, allograft human ADSs were transplanted. Group 3 was subjected to PBM (wavelength: 890 nm, peak power output: 80 W, pulse frequency: 80 Hz, pulsed duration: 180 ns, duration of exposure for each point: 200 s, power density: 0.001 W/cm2, energy density: 0.2 J/cm2) immediately after surgery, which continued for 6 days per week for 16 days. Group 4 received both the human ADS and PBM. In addition, we inflicted an ischemic, delayed healing, and infected wound simulation in all of the rats. The wounds were infected with methicillin-resistant Staphylococcus aureus (MRSA).
Results All three treatment regimens significantly decreased the amount of microbial flora, significantly increased wound strength and significantly modulated inflammatory response and significantly increased angiogenesis on day 16. Microbiological analysis showed that PBM+ADS was significantly better than PBM and ADS alone. In terms of wound closure rate and angiogenesis, PBM+ADS was significantly better than the PBM, ADS and control groups.
Conclusions Combination therapy of PBM+ADS is more effective that either PBM or ADS in stimulating skin injury repair, and modulating inflammatory response in an MRSA-infected wound model of rats with DM1.
- wound healing
Data availability statement
Data are available upon reasonable request.
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Data availability statement
Data are available upon reasonable request.
Supplementary materials
Supplementary Data
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Footnotes
Correction notice This article has been corrected since it was published. Dr. Abdollah Amini is added as co-corresponding author.
Contributors MB is the guarantor of this work, and researched data and wrote the manuscript. SC, AA, and SMH contributed to the conception and design. RE-M, FZ, AM, SD, ND, MR, MH, and HA performed the experiments and collection of data and SB primary edited the manuscript. SKG contributed to the data analysis and interpretation. SC and SS edited scientifically the manuscript.
Funding This study was financially supported by the Research Department of the School of Medicine at Shahid Beheshti University of Medical Sciences (grant no. 14601). SC was supported in part by NIH grant DK105692.
Competing interests None declared.
Provenance and peer review Not commissioned; externally peer reviewed.