Article Text
Abstract
Introduction Obesity is a risk factor for type 2 diabetes mellitus (T2DM) and cardiovascular disease. T2DM increases the risk of cardiovascular-related death. We investigated changes in circulating exosomal microRNA (miRNA) profiles in patients with DM with obesity compared with patients without DM with obesity.
Research design and methods This prospective study involved 29 patients with obesity (patients without DM=16, patients with DM=13) and healthy volunteers (HVs) (n=18). We measured circulating levels of exosomal miRNAs by next-generation sequencing and compared miRNA levels across the three groups.
Results The expression levels of 25 miRNAs (upregulated=14, downregulated=11) differed between patients with obesity with DM and patients with obesity without DM. Compared with HV, patients with DM with obesity had 53 dysregulated miRNAs. Additionally, moving stepwise from HV to patients with obesity without DM to patients with obesity with DM, there was a consistent increase in expression levels of miR-23a-5p and miR-6087 and a consistent decrease in expressions levels of miR-6751-3p.
Conclusions Our data show that the exosomal miRNAs is altered by dysregulated glucose metabolism in patients with obesity. This circulating exosomal miRNA signature in patients with obesity with or without DM is a potential biomarker and therapeutic target in these patients.
- obesity
- diabetes mellitus
- type 2
- biomarkers
- transcription
- genetic
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Footnotes
HyosK and Y-UB contributed equally.
Contributors HyosK contributed to data analysis, statistical analysis, literature search and writing the manuscript. Y-UB involved in data analysis, conduction of the experiments, statistical analysis and cowriting the manuscript. HL, HyouK, JSJ, HN, DCH and DWB involved in data analysis, searching literature and editing the manuscript. SaHK and HKP involved in study design, data collection and editing the manuscript. SR and SoHK contributed to study design, submitting the grant, conduction of the experiments, data collection and analysis, literature search and editing the manuscript.
Funding This study was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MSIT) (NRF-2019M3E5D3073092) and the Soonchunhyang University Research Fund (20191124).
Competing interests None declared.
Patient consent for publication Not required.
Ethics approval This study was conducted in accordance with the Declaration of Helsinki, and the study protocol was approved by the institutional review board of Soonchunhyang University Seoul Hospital (IRB No: 2015-11-020).
Provenance and peer review Not commissioned; externally peer reviewed.
Data availability statement Data are available on reasonable request.