PT  - JOURNAL ARTICLE
AU  - Cécilia Légaré
AU  - Véronique Desgagné
AU  - Cédrik Poirier
AU  - Kathrine Thibeault
AU  - Frédérique White
AU  - Andrée-Anne Clément
AU  - Michelle S Scott
AU  - Pierre-Étienne Jacques
AU  - Patrice Perron
AU  - Renée Guérin
AU  - Marie-France Hivert
AU  - Luigi Bouchard
TI  - First trimester plasma microRNAs levels predict Matsuda Index-estimated insulin sensitivity between 24th and 29th week of pregnancy
AID  - 10.1136/bmjdrc-2021-002703
DP  - 2022 Mar 01
TA  - BMJ Open Diabetes Research & Care
PG  - e002703
VI  - 10
IP  - 2
4099  - http://drc.bmj.com/content/10/2/e002703.short
4100  - http://drc.bmj.com/content/10/2/e002703.full
SO  - BMJ Open Diab Res Care2022 Mar 01; 10
AB  - Introduction Gestational diabetes mellitus (GDM) is a consequence of an imbalance between insulin sensitivity (IS) and secretion during pregnancy. MicroRNAs (miRNAs) are small and secreted RNA molecules stable in blood and known to regulate physiological processes including glucose homeostasis. The aim of this study was to identify plasmatic miRNAs detectable in early pregnancy predicting IS at 24th-29th week of pregnancy.Research design and methods We quantified circulating miRNAs in 421 women in plasma collected at 9.6±2.2 weeks of pregnancy using next-generation sequencing.Results we detected 2170 miRNAs: 39 (35 positively and 4 negatively) were associated with IS as estimated by the Matsuda Index at 26.4±1.0 weeks of pregnancy. Lasso regression identified 18 miRNAs independently predicting Matsuda Index-estimated IS. Together with gestational age, maternal age and body mass index at first trimester, they explain 36% of IS variance in late second trimester of pregnancy. These miRNAs regulate fatty acid biosynthesis and metabolism among other pathways.Conclusions In summary, we have identified first trimester plasmatic miRNAs predictive of Matsuda Index-estimated IS in late second trimester of pregnancy. These miRNAs could also contribute to initiate and support IS adaptation to pregnancy potentially through lipid metabolism regulation.Data are available on reasonable request. The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.