Table 1

Custom-built multiphoton microscopy system specifications

ModalityPurposeLight source
(nm)
Emission
(nm)
Power
(mW)
Field of view
(number of pixels; µm)
Optical coherence tomography angiography (OCTA)Angiogenesis850±6015(1000×1000);
(3900×3900)
Fluorescence lifetime imaging microscopy (FLIM)NAD(P)H lifetime750450±2020(512×512);
(180×180)
Two-photon excitation fluorescence (2PEF)NAD(P)H intensity750450±2020(512×512);
(180×180)
FAD intensity950550±2025(512×512);
(180×180)
Second harmonic generation (SHG) microscopyCollagen deposition104052025(4628, 4628);
(3200×3200)
Color image using a CCD cameraVisual examinationWhite LED light(261, 261);
(4200×4200)
  • CCD, charge-coupled device; FAD, flavin adenine dinucleotide; LED, light-emitting diode; NAD(P)H, nicotinamide adenine dinucleotide (phosphate).