Cell culture conditions
| Cell group names | Glucose (mM) | FC (μM) | DFO (μM) | NAC (μM) | BAY 11-7082 (mM) | |
| 1 | LG | 5.5 | ||||
| 2 | HG | 30 | ||||
| 3 | LG+FC | 5.5 | 800, 1600, 2400 | |||
| 4 | HG+FC | 30 | 800, 1600, 2400 | |||
| 5 | HG+DFO | 30 | 2400 | |||
| 6 | HG+FC+DFO | 30 | 2400 | 2400 | ||
| 7 | HG+NAC | 30 | 2400 | |||
| 8 | HG+FC+NAC | 30 | 2400 | 2400 | ||
| 9 | HG+NF-κB-I | 30 | 10 | |||
| 10 | HG+FC+NF-κB-I | 30 | 2400 | 2400 | 10 |
HUVEC cells were cultured in 10 groups.
DFO, deferoxamine; FC, ferritin citrate; HG, high glucose; HUVEC, human umbilical vein endothelial cell; LG, low glucose; NAC, N-acetyl-L-cysteine; NF-κB-I, nuclear factor-κB inhibitor.