Risk of bias and reporting quality assessment using the ARRIVE guidelines
| Criteria | Study | |||||||
| 14 | 15 | 16 | 17 | 18 | 19 | 20 | ||
| Method | ||||||||
| Ethical statement | Reported the ethics committee approval | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Study design | Reported control vs Ischemia group | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Animal characteristics | Animal age/weight | 1 | 0 | 1 | 1 | 0 | 1 | 1 |
| Sex | 1 | 0 | 1 | 1 | 0 | 1 | 1 | |
| Strain | 1 | 1 | 1 | 1 | 0 | 1 | 1 | |
| Experimental procedures | Detailed the ischemia protocol | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Detailed the wound development protocol | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| Animal randomization to different groups | Indicated methods employed to minimize selection bias between groups | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| Statistics | Reported the statistical methods used for each analysis | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Experimental measurements | Reported the process employed for measurement of wound area | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Reported that the wound measurements were performed in duplicates or blinded to confirm reliability | 0 | 1 | 1 | 0 | 0 | 0 | 0 | |
| Results | ||||||||
| Baseline data | Reported baseline data for all animal groups | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Numbers analyzed | Reported the absolute number of animals used in each group | 1 | 0 | 1 | 1 | 1 | 1 | 1 |
| Data estimation | Reported the results of analysis with a measure of precision (SD or SEM) | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Adverse events | Reported if there was no/any adverse events or infection arising from the wounds | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Score of 15=100% | 12 | 10 | 14 | 12 | 9 | 12 | 12 | |
| Total score (%) | 80 | 66.6 | 93.3 | 80 | 60 | 80 | 80 | |
1=Yes; 0=No.
ARRIVE, Animal research: Reporting of In Vivo Experiments.