Summary
Eight streptozotocin-injected Wistar rats and eight controls were fixed by whole-body perfusion 4 months after beginning of the experiment, the nervus radialis was dissected and processed for light and electron microscopy. After light-microscopic study standard photographs of nerve cross sections were measured by means of a semiautomatic image analyzer. The following measurements were obtained: (1) surface of fibres, axons, and myelin sheaths, (2) ratio of myelin to axon surface, and (3) percent of endoneural space. Group means and standard deviations were calculated, and cumulated size class distributions were made. Representative nerve specimens from all animals were also studied by electron microscopy. The quantitative study revealed in the diabetics a severe reduction of the average myelin surface, a mild increase of axonal cross section and of endoneural space, a reduction of myelin/axon ratio and a mild reduction in cross section of the nerve. Ultrastructural lesions of minor degree were found in the cytoplasm of Schwann and mesenchymal cells, no lesion was observed in axons. These findings demonstrate the presence of neuropathy 4 months after induction of diabetes and support the pathogenetic role of the Schwann cell in our experimental model.
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References
Clements RS Jr (1979) Diabetic neuropathy. New concepts of its etiology. Diabetes 28:604–611
Jakobsen J (1976a) Axonal dwindling in early experimental diabetes. I. A study of cross-sectioned nerves. Diabetologia 12:539–546
Jakobsen J (1976b) Axonal dwindling in early experimental diabetes. II. A study of isolated nerve fibers. Diabetologia 12:547–553
Jakobsen J, Lundbaek K (1976) Neuropathy in experimental diabetes: an animal model. Br Med J 2:278–279
Waber S, Meister V, Rossi GL, Mordasini R-C, Riesen WF (1981) Studies on retinal microangiopathy and coronary macroangiopathy in rats with streptozotocin-induced diabetes. Virchows Arch B Cell Pathol (in press)
Zemp C, Bestetti G, Rossi GL (1981) Morphological and morphometric study of peripheral nerves from rats with streptozotocin-induced diabetes mellitus. Acta Neuropathol (Berl) 53:99–106
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Supported by the Schweizer Nationalfonds grants nos. 3.198-0.77 and 3.552-0.79
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Bestetti, G., Rossi, G.L. & Zemp, C. Changes in peripheral nerves of rats four months after induction of streptozotocin diabetes. Acta Neuropathol 54, 129–134 (1981). https://doi.org/10.1007/BF00689405
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DOI: https://doi.org/10.1007/BF00689405