Biochemical engineering
Mass production of d-psicose from d-fructose by a continuous bioreactor system using immobilized d-tagatose 3-epimerase

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Abstract

An improved process for the mass production of d-psicose from d-fructose was developed. A d-fructose solution (60%, pH 7.0) was passed at 45°C through a column filled with immobilized d-tagatose 3-epimerase (d-TE) which was produced using recombinant Escherichia coli, and 25% of the substrate was converted to d-psicose. After epimerization, the substrate, d-fructose, was removed by treatment with baker's yeast. The supernatant was concentrated to a syrup by evaporation under vacuum and d-psicose was crystallized with ethanol. Approximately 20 kg of pure crystal d-psicose was obtained in 60 d.

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