Rhinitis, sinusitis, and upper airway diseaseGlandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps
Section snippets
Patients
Patients with CRS were recruited from the allergy and otolaryngology clinics at Northwestern University and the Northwestern Sinus Center. Sinonasal and polyp tissues were obtained from routine functional endoscopic sinus surgery in patients with CRS. All subjects met the criteria for CRS as defined by the Sinus and Allergy Health Partnership.1
Patients with an isolated antrochoanal polyp, cystic fibrosis, or unilateral NPs were excluded from the study. Details of subjects' characteristics are
Mast cell expression and distribution in patients with CRS
Sinonasal and polyp tissues were collected from 70 subjects with CRSsNP, 91 subjects with CRSwNP, and 42 control subjects to determine the levels of expression of mast cells and their products in patients with CRS. Subject characteristics are shown in Table I.
To estimate the levels of mast cells in nasal mucosa, we first assessed the expression of mRNA for tryptase in UT from patients with CRSsNP, patients with CRSwNP, and controls, as well as in NPs from patients with CRSwNP. The expression of
Discussion
The current study provides the first in-depth description of the expression, distribution, and phenotype of mast cells in NPs from patients with CRSwNP. We found increased levels of mast cells in NPs than in UT from control subjects and patients with CRS (Fig 1). We made the striking observation that mast cells were present within glandular tissues contained in NPs but were almost never detected within glands in UT from either patients or controls. Another important original finding was that
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This study was supported by National Institutes of Health grants R37HL068546-27, R01HL078860, and RO1AI072570 and the Ernest S. Bazley Trust.
Disclosure of potential conflict of interest: R.C. Kern and R.P. Schleimer received research support from the National Institutes of Health. The rest of the authors declare that they have no relevant conflicts of interest.