ArticleRobust global microRNA expression profiling using next-generation sequencing technologies
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microarray
microRNAs
NanoString
next-generation sequencing
quantitative real-time PCR
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Supplementary Information accompanies the paper on the Laboratory Investigation website
This study outlines the strengths and pitfalls of several miRNA profiling technologies, including a microarray platform, next-generation sequencing (NGS) technologies and the NanoString nCounter System. Overall, NGS has the greatest detection sensitivity, largest dynamic range of detection and highest accuracy in differential expression analysis when compared to gold-standard quantitative real-time PCR. Expression profiles between paired frozen and FFPE samples are similar.
Supplementary information The online version of this article (doi:10.1038/labinvest.2013.157) contains supplementary material, which is available to authorized users.
© 2014 United States & Canadian Academy of Pathology.