Basic-alimentary tractA novel, noninvasive method for the measurement of intestinal fat absorption☆
Section snippets
Diet preparation
Olestra and SPB (SEFOSE-2275) were gifts from Procter & Gamble. The fatty acids in olestra (percentage weight) is as follows: palmitic, 22.3%; stearic, 3.7%; oleic, 27.5%; linoleic, 32.9%; behenic 5.1%. The behenic acid in this olestra was included with that of SPB in all calculations in studies 1, 2, 4, and 5. The fatty acid composition of the SPB was as follows: palmitic, 2.0%; stearic, 3.1%; oleic, 7.1%; linoleic, 2.9%; arachidic, 5.1%; behenic, 77.0%. The calcium salts of stearic (63.8%)
Study 1: olestra in mice
The behenic acid in the feces collected from the safflower oil-fed animals (diet S) was in the range of 33.5%–66.4% of the total fatty acids. In this same time period, behenic acid accounted for 12.5%–19.3% of the fatty acids in the feces from the animals fed olestra and safflower oil (diet O21).
The absorption of safflower oil by the mice was calculated to be 95.9% ± 0.5% of that which was fed, based on the 17 fecal samples that were collected over the 3 days. The analyses of individual samples
Discussion
We have found that SPB is a suitable marker for the measurement of the absorption of dietary fat. It is nontoxic, commercially available, and readily measured by standard gas chromatographic methods. It was found to be a satisfactory marker for both unhydrolyzed fat (olestra) and hydrolyzed fat (calcium soaps).
Our data suggest a standard protocol for the measurement of fat in rodents, consisting of a diet containing fat and SPB for 2 nights with collection of fecal samples after the second
References (13)
- et al.
Effects of partial replacement of dietary fat by olestra on dietary cholesterol absorption in man
Metabolism
(1990) - et al.
Physical properties of pure sucrose octaesters
Chem Phys Lipids
(1978) - et al.
Disposition of ingested olestra in the Fischer 344 rat
Fundam Appl Toxicol
(1995) - et al.
Short-term study of sucrose polyester, a nonabsorbable fat-like material, as a dietary agent for lowering plasma cholesterol
Am J Clin Nutr
(1976) - et al.
Olestra ingestion and dietary fat absorption in humans
J Nutr
(1997) - et al.
Fecal triglyceride excretion is not excessive in pancreatic insufficiency
Gastroenterology
(1989)
Cited by (0)
- ☆
Supported by a PNF Pilot and Feasibility Grant funded by the National Institutes of Health (DK59630) and by RO1 DK 56910 (to P.T.).