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Research Methods & Reporting

CONSORT 2010 statement: extension to randomised crossover trials

BMJ 2019; 366 doi: https://doi.org/10.1136/bmj.l4378 (Published 31 July 2019) Cite this as: BMJ 2019;366:l4378
  1. Kerry Dwan, statistical editor1,
  2. Tianjing Li, associate professor2,
  3. Douglas G Altman, professor of statistics in medicine3,
  4. Diana Elbourne, professor of healthcare evaluation4
  1. 1Review Production and Quality Unit, Editorial and Methods Department, Cochrane Central Executive, Cochrane, St Alban’s House, London SW1Y 4QX, UK
  2. 2Center for Clinical Trials and Evidence Synthesis, Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA
  3. 3Centre for Statistics in Medicine, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, UK
  4. 4London School of Hygiene and Tropical Medicine, Department of Medical Statistics, London, UK
  1. Correspondence to: K Dwan kdwan{at}cochrane.org
  • Accepted 7 June 2019

Evidence shows the quality of reporting of randomised controlled trials is not optimal. The lack of transparent reporting impedes readers from judging the reliability and validity of trial findings, prevents researchers from extracting information for systematic reviews, and results in research waste. The Consolidated Standards of Reporting Trials (CONSORT) statement was developed to improve the reporting of randomised controlled trials. The primary focus of the statement was on parallel group trials with two treatment groups. Crossover trials are a particular type of trial for chronic conditions in which participants are randomised to a sequence of interventions. They are a useful and efficient design because participants act as their own control. However, the reporting of crossover trials has been variable and incomplete, which hinders their use in clinical decision making and by future researchers. We present the CONSORT extension to randomised crossover trials, which aims to facilitate better reporting of crossover trials. The CONSORT 2010 checklist is revised for crossover designs, and introduces a modified flowchart and baseline table to enhance transparency. Examples of good reporting and evidence based rationale for CONSORT crossover checklist items are provided.

Summary points

  • The Consolidated Standards of Reporting Trials (CONSORT) statement provides a minimum set of 25 items to be reported with rationale and exemplars for all randomised trials

  • CONSORT extension to crossover trials extends 14 items of the CONSORT statement

  • The use of the CONSORT extension to crossover trials will improve reporting of randomised crossover trials

Inadequate reporting of randomised controlled trials (RCTs) is associated with bias in the estimation of treatment effects12; it also impairs the critical appraisal of the quality of randomised trials, which is important when assessing the validity of the results of the individual trial and when conducting systematic reviews. To attempt to address this issue, the Consolidated Standards of Reporting Trials (CONSORT) statement was developed and includes a set of recommendations for the reporting of RCTs.3 The statement comprises a checklist of essential items that should be included in reports of RCTs and a diagram to document the flow of participants through the trial from before group assignment through to the final analysis. These items are evidence based when possible. An explanation and elaboration of the rationale for the checklist items are provided in an accompanying article.4 Many journals now require that reports of RCTs conform to the recommendations in the CONSORT statement.5

The primary focus of the CONSORT statement is the most common type of RCT with two treatment groups (two “arms”) using an individually randomised, parallel group, superiority design.3 Almost all the elements of the CONSORT statement apply equally to RCTs with other designs, but some elements need adaptation, and in some cases additional issues need to be discussed. Members of the CONSORT group have published several extension papers that augment the CONSORT statement for different types of interventions and data. Extensions of CONSORT 2010 to different trial designs have also been published, including cluster randomised trials,6 non-inferiority and equivalence trials,7 N-of-1 trials,8 pragmatic trials,9 and within person trials.10 As part of that series, in this paper we extend the CONSORT 2010 recommendations to simple crossover RCTs in which participants receive two treatments sequentially over two periods and the order in which treatments are received is randomised.

Scope of this paper

Firstly, we summarise the key methodological features of crossover trials. Secondly, we consider the empirical evidence about how common crossover trials are and review published studies about the quality of reporting of such trials. After these literature reviews, we make suggestions for amendments to the CONSORT checklist adapted for crossover trials and give illustrative examples of good reporting. In this guideline we focus on the simplest and most common form of the randomised crossover trial in which all participants receive two interventions in one of two sequences (known as the 2×2 or AB/BA design). Most of the recommendations also apply to the more complicated designs (more than two interventions, periods, or sequences). In a separate section, we briefly discuss specific issues that arise in trials comparing more than two interventions.

Methodological features of randomised crossover trials

In contrast to a parallel group trial, each individual in a crossover trial receives multiple interventions but in a random order; that is, participants are randomised to sequences of interventions. In this way, each participant acts as his or her own control. Such prespecified designs should not be confused with trials in which some individuals “cross over” through non-compliance or use of rescue medication, or in which all participants in the control group are given the chance to “cross over” to the experimental treatment at the end of the main trial. Zeng and colleagues found that almost one quarter of records (n=17/72) labelled as “crossover assignment” did not use a randomised crossover design to randomise participants to a sequence; instead, these trials allowed participants to change intervention during the course of the trial.11

Randomised crossover trials present particular challenges. One challenge is the potential for a “carry over effect”; that is, the effect of the first intervention persists into the second period so that the observed difference between the treatments depends on the order in which they were received (see box 1 for glossary of terms). A carry over effect could have a range of causes. In addition to the obvious problem of a drug or other treatment remaining in the system, participants’ later responses can be affected by previous side effects or other reactions to previous treatment. It is recommended that crossover trials should include a sufficient “washout” between the end of the first intervention and the start of the second intervention, so that any effects from the first intervention will not be “carried over” to the measurement of outcome in the second intervention period.

Box 1

Glossary

Period: a length of time when one treatment was received.

Sequence: treatment sequence (AB, BA), participants allocated to the AB study arm receive treatment A first, followed by treatment B, and vice versa in the BA arm.

Within participant variability: the expected standard deviation of the within participant differences.

Washout: a length of time between treatment periods when no treatment is received to allow the treatment to wear off.

Carry over effect: when the effect of the first intervention persists into the second period.

Period effect: the outcome of interest changes with time irrespective of treatment effect.

Within participant comparison: a within participant comparison takes into account the correlation between measurements for each participant because they act as their own control, therefore measurements are not independent.

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Another issue is the “period effect,” which occurs when the outcome of interest changes with time irrespective of treatment effect; for example, the condition might not be stable or the effect of treatment is seasonal.

A further issue is the possibility of participants dropping out of the trial if the first intervention is either very successful or unsuccessful; the results for these participants cannot be included in the analysis.

Design

The particular strength of the simple AB/BA crossover design is that both interventions are evaluated using the same participant, which allows comparison at the individual rather than the group level. In addition, participants in a crossover trial can express preferences by comparing their experiences of the two interventions, which is not possible in a parallel group design because participants will only receive one intervention.12

A crucial methodological question is whether the use of the crossover design is justified. Crossover trials are most appropriate for symptomatic treatment (that is, treatment for symptoms, such as pain) of conditions or diseases that are chronic or relatively stable (for example, multiple sclerosis or rheumatoid arthritis), at least over the time period under study; additionally, when the treatment effects are reversible and short lived. The crossover design is inappropriate when the condition of interest can be cured or when participants will probably die during the trial period. The design is commonly used, however, in less appropriate circumstances. For example, pregnancy is an intended outcome of subfertility treatment. If a woman becomes pregnant during the first period of the trial (that is, before crossover), she will be excluded from subsequent phases of the trial. Nevertheless the crossover design is defended in the field13 (for instance, it has been suggested that pregnancies can be treated statistically as “missing at random”14), and remains common despite criticism.15

The sample size calculation for such trials is based on the within participant variability in responses. The crossover design is much more efficient than the parallel design when there is a high positive correlation between participants’ responses to the different treatments. Compared with a parallel group design, fewer participants are required for a crossover trial to obtain the same power for a target effect size and type 1 error rate.

Crossover trials have certain weaknesses. In particular, there can be carry over effects as previously discussed. Participants could drop out after the first treatment and so not receive the second treatment. Withdrawal might be related to side effects.

Analysis

The analysis of a crossover trial should be based on paired data.161718 The estimation approaches should account for the correlation of repeated measurements in the same individual. The tests for significance should use procedures such as the paired t test (assuming no carry over or period effect), which is based on within participant differences for a continuous response and the Mainland-Gart test for a binary response.1920

A previously recommended but criticised method for analysing crossover trials was to test for carry over, and if this was statistically significant, to discard the second period data and analyse only the data from the first period. In other words, the first period’s data are analysed as if they were from a parallel group trial. Freeman21 showed that this strategy is flawed and leads to biased answers (which is generally the case when the choice between two analyses is based on the result of a preliminary hypothesis test). Senn17 and others have argued that the use of the two period, two treatment crossover design is effectively built on the assumption that there is minimal carry over effect.

The other statistical issue specific to crossover studies is the need for adjustment for possible period and carry over effects. Parameters can be included for carry over effect in the statistical model. In the AB/BA crossover design, the terms “carry over” and “treatment by period” interaction are sometime used interchangeably because the effects of “carry over” and “treatment by period interaction” are not separately identifiable in the data. Although the carry over effect can be estimated, Senn17 and others have argued that there is little value in using the carry over effect to adjust the treatment effect. This is because such adjustment relies on assumptions about the nature of the possible carry over effect and reduces the statistical efficiency for estimating the main treatment effect.

A period effect can be dealt with and adjusted for in the analysis. In the AB/BA crossover design, when equal numbers of participants are allocated to each sequence, then on average the period effect will not bias the estimate of treatment effect. However, a period effect will affect the variance estimate because it interferes with how much of the treatment effect might be attributed to random variation. It is important for authors to present data to help readers understand the extent of the period effect and communicate clearly whether the period effect was adjusted for or not adjusted for in the analysis, and whether such a decision was made a priori.

How common are randomised crossover trials?

A detailed review of all PubMed indexed RCTs published in December 2000 found that 74% (383/519) of trials used a parallel design and 22% (116/519) were crossover trials.22 Of the trials indexed in Medline in December 2000, 22% (116/526) were crossover trials and most used two treatments (72%) and had two periods (64%).23 A review of all PubMed indexed RCTs published in December 2006 found 77% (477/616) of trials used a parallel design and 16% (100/616) were crossover trials.24 A review of intervention studies registered with ClinicalTrials.gov between 2007 and 2010 found that 11.2% (4351/38 969) were crossover trials.25 A more recent review of PubMed, in December 2012, found that 8.7% (98/1122) of RCTs had a crossover design.26

What is the quality of reporting of randomised crossover trials?

Although articles on the quality of reporting of RCTs in relation to CONSORT are relatively common, few articles have specifically examined the quality of reporting of crossover trials. Mills and colleagues found that randomised crossover trials indexed in Medline in December 2000 frequently omitted details on design, analysis, and interpretation.23 However, most trials reported and defended a washout period (69%, 87/127) and reported use of paired data in the analysis (95%, 121/127). Gewandter and colleagues investigated 124 crossover clinical trials of drug treatments for chronic pain published between 1993 and 2013. They found that 28% (35/124) of trials reported baseline and post washout pain levels, and only 31% (23/75) reported a sample size calculation that specifically indicated that it was based on within participant variability.27 Straube and colleagues considered 98 crossover trials on chronic painful conditions published between 1990 and 2014 and indexed on PubMed. They found that adverse events were poorly reported in the abstracts of the trial reports and also infrequently reported in the full article, and only 23% (23/98) presented a breakdown by treatment period.28 Zeng and colleagues found that of 54 phase III randomised crossover trials analysed from ClinicalTrials.gov in September 2014, nearly two thirds had a simple AB/BA design, with most trials (87%, 47/54) providing sufficient information for the participant flow throughout the trial.11 Baseline characteristics were most often reported for all participants as a single group (59%, 32/54), and primary outcomes and adverse events were most commonly reported “per intervention” (81%, 44/54 and 83%, 45/54, respectively). The reporting of results in baseline characteristics, outcome measures, and adverse events generally did not appear to fully reflect the crossover design.

Several studies have considered the reporting of randomised crossover trials in relation to meta-analyses.293031 They found that data were frequently reported inappropriately to allow them to be included in a meta-analysis.

These studies show that the problems have not improved over several years and most of these studies call for guidance on reporting of randomised crossover trials.

Methods used to develop this CONSORT extension

In May 2002, several CONSORT authors met in Arlington, Virginia, USA to consider extensions to the 2001 CONSORT statement for a range of different designs. The first drafts of a paper extending the statement to crossover trials were developed by Doug Altman and Diana Elbourne in 2002-03. In 2010, the CONSORT statement was updated. Work on the extension to crossover trials progressed in 2014 when Kerry Dwan and then Tianjing Li joined the group. The checklist and explanatory text were informed by reviews of published randomised trials (as cited above) and completed through numerous teleconferences between the authors from 2014 to 2018. We followed guidance of the CONSORT group to include a member of the CONSORT Group Executive (Doug Altman), who was also chair of the EQUATOR Steering Group. A draft paper was distributed to the wider CONSORT group and other selected individuals, and the paper was revised to take account of their feedback, and approved by the Executive.

CONSORT checklist for randomised crossover RCTs

We discuss the checklist items and focus on any changes to the standard CONSORT items for randomised crossover trials. We explain the background, and provide one or more examples of good reporting. We also discuss other checklist items for which we do not suggest any modifications but where implementation requires specific considerations for crossover RCTs. Table 1 shows the suggested modifications to the standard CONSORT checklist for randomised crossover trials.

Table 1

CONSORT checklist of information to include when reporting randomised crossover trials

View this table:

Title and abstract

Item 1a: Title

Identification as a randomised crossover trial in the title.

Standard CONSORT item—Identification as a randomised trial in the title.

Example 1—“Effect of Ginkgo biloba on visual field and contrast sensitivity in Chinese patients with normal tension glaucoma: a randomized, crossover clinical trial”.33

Example 2—“Effects of unfermented and fermented whole grain rye crisp breads served as part of a standardized breakfast, on appetite and postprandial glucose and insulin responses: a randomized cross-over trial”.34

Explanation—The primary reason for identifying the design in the title is to help readers to identify the study design. Identification of the trial as a randomised crossover trial also ensures that readers will start thinking of the implications of the design in relation to sample size and analysis.

Item 1b: Abstract

Specify a crossover design and report all information outlined in table 2.

Table 2

Information to include in abstract of report of randomised crossover trial: extension of CONSORT for abstracts checklist35

View this table:

Standard CONSORT item—Structured summary of trial design, methods, results, and conclusions (for specific guidance see CONSORT for abstracts3).

Example

CONTEXT: The relationship between sildenafil citrate use and reported adverse cardiovascular events in men with coronary artery disease (CAD) is unclear.

OBJECTIVE: To evaluate the cardiovascular effects of sildenafil during exercise in men with CAD.

DESIGN, SETTING, AND SUBJECTS: Randomised, double blind, placebo controlled two period crossover trial conducted March to October 2000 at a US ambulatory care referral centre among 105 men (55 to receive sildenafil first, and 55 to receive placebo first) with a mean (SD) age of 66 (9) years who had erectile dysfunction and known or highly suspected CAD.

INTERVENTIONS: All patients underwent two symptom limited supine bicycle echocardiograms separated by an interval of one to three days after receiving a single dose of sildenafil (50 or 100 mg) or placebo one hour before each exercise test.

MAIN OUTCOME MEASURES: Haemodynamic effects of sildenafil during exercise (onset, extent, and severity of ischemia) assessed by exercise echocardiography.

RESULTS: The difference between mean change after sildenafil and placebo use was 4.3 (95% CI 0.9 to 7.7; P=0.01). Exercise capacity was similar with sildenafil use and placebo use (mean difference 0.07; 95% CI −0.06 to 0.19; P=0.29). Exercise blood pressure and heart rate increments were similar. Dyspnoea or angina developed in 69 patients who took sildenafil and 70 patients who took placebo (P=0.89); exercise electrocardiography was positive in 12 patients (11%) who took sildenafil and 17 patients (16%) who took placebo (P=0.09). Exercise induced wall motion abnormalities developed in similar numbers of patients after sildenafil and placebo use (84 and 86 patients, respectively; P=0.53). Wall motion score index at peak exercise was similar after sildenafil and placebo use (mean difference 0.01; 95% CI −0.01 to 0.03; P=0.40).

CONCLUSION: In men with stable CAD, sildenafil had no effect on symptoms, exercise duration, or presence or extent of exercise induced ischaemia, as assessed by exercise echocardiography. (Adapted from Arruda-Olson and colleagues.36)

Explanation—Clear, transparent, and sufficiently detailed abstracts are important. Readers might only have access to the abstract, and many others will skim it before deciding whether to read further. A well written abstract also helps retrieval of relevant reports from electronic databases. In 2008 a CONSORT extension on reporting abstracts of randomised trials was published,35 and those recommendations were incorporated into CONSORT 2010.3 Abstracts for crossover RCTs should indicate the design of the trial and therefore the randomisation to sequence and analysis by taking into account the within participant comparisons. Table 2 shows information to be included in the abstract of a crossover trial.

We were not able to find examples of good reporting tackling all the items required. We have therefore adapted a published abstract (see example).

Methods

Item 3a: Trial design

Rationale for a crossover design. Description of the design features including allocation ratio, especially the number and duration of periods, duration of washout period, and consideration of carry over effect.

Standard CONSORT item—Description of trial design (such as parallel, factorial) including allocation ratio.

Example 1—“The trial was a randomised double-blind, placebo controlled, crossover design of 15 months’ duration … randomisation (1 month); treatment period one (6 months); washout (2 months); and finally treatment period two (6 months) … Patients were randomly assigned azithromycin in treatment period one, followed by placebo in treatment period two, or placebo in treatment period one followed by azithromycin in treatment period two.”37

Example 2—“A crossover design was chosen for this study instead of the more traditional randomized, parallel-group design because the within-patient variation is less than the between patient variation and thus required fewer patients. In addition, some of the known disadvantages of the crossover design (e.g. larger dropout rate, instability of the patient’s condition, and a potential carryover effect) were not expected in this study.”38

Example 3—“Each treatment period was separated by a 2-week washout, equating to five or more half-lives for either treatment, to allow the effective systemic elimination of the drug before initiation of subsequent treatment.”39

Example 4—“We did not include a medicine-free period between treatments to increase patient safety. In addition, we believed the 8-week treatment period was sufficient to allow for the washout of the first treatment before the efficacy measurements at the end of period 2.”40

Explanation—The methods should contain a rationale for the use of a crossover design in the given setting. In particular, given that a carry over effect can neither be identified with sufficient power, nor can adjustment be made for such an effect in the 2×2 crossover design, the assumption needs to be made that any carry over effects are negligible and some justification presented for this. The description of the design should make clear how many interventions were tested, through how many periods, including information on the length of the treatment, run in, and washout periods (if any).

Item 3b: Changes to methods

Important changes to methods after trial commencement (such as eligibility criteria), with reasons.

No change from standard CONSORT item.

Explanation—A test for carry over is not recommended. However, if a test for carry over is performed and as a result the authors use only the first period data, then this should be reported. The use of the test should also be discussed under item 12a (Statistical methods). The reason for the presence of a carry over should also be discussed.

Item 5: Interventions

The interventions with sufficient details to allow replication, including how and when they were actually administered.

Standard CONSORTitem—The interventions for each group with sufficient details to allow replication, including how and when they were actually administered.

Explanation—For this item, “for each group” was deleted for the extension as in a 2×2 randomised crossover trial; the intention is that all participants receive both of the interventions.

Item 7a: Sample size

How sample size was determined, accounting for within participant variability.

Standard CONSORT item—How sample size was determined.

Example—“Earlier research of the Cambridge study site (unpublished data) with the Apathy Evaluation Scale [AES] showed a mean score of 31 points (standard deviation SD=15.6). If we define a clinical significant improvement on the AES-I as a 35% reduction of the mean score, this leads to an absolute effect size of 0.35*31 points=10.85 points. Thus a conservative estimate of 10 units is used for sample size estimation. Furthermore a within subjects SD=15.0 is assumed. When the sample size in each sequence group is 19, (a total sample size of 38) a 2×2 crossover design will have 80% power to detect a difference in means of 10.000 (the difference between a Treatment 1 mean, µ1, of 31 and a Treatment 2 mean, µ2, of 21 ) assuming that the crossover ANOVA [analysis of variance] √MSE [mean square error] is 15.000 (the Standard deviation of differences, sd, is 21.213) using a two group t test (Crossover ANOVA) with a 0.050 two-sided significance level. In order to account for potential drop-outs 40 patients will be randomized. Sample size calculation was performed with nQuery 7.0.”41

Explanation—A key advantage of the crossover design is that, for a given significance level, power, and effect size, a smaller sample size is required compared with a parallel design in which each participant receives only one treatment. This is because each participant acts as his or her own control (each participant receives the experimental and control intervention), so the within participant variability is removed.

It is important that trial authors report the usual quantities required for sample size calculation, including significance level and power, but also for continuous variables the within participant variability as shown in the example. It is often difficult to get the necessary within participant information to inform the sample size calculation. Published reports of crossover trials should clarify how the sample size was determined, and ideally should indicate that an appropriate estimate of within participant variability was used. For crossover trials with a continuous outcome, it is the expected standard deviation of the within participant differences that must be incorporated into the sample size estimation. In practice, for many trials it is unlikely that there will be data to support a realistic estimate of this value; however, ignoring it could result in an overestimation of the sample size for a crossover trial and is thus conservative.42 Some attempt should be made to estimate the standard deviation of the within participant differences (or allow for the correlation).

Likewise, with a binary outcome, not considering the paired nature of the data will result in an unnecessarily large sample size due to failure to account for the within participant comparison arising from the paired design. Authors are expected to give appropriate details so that the sample size calculation can be replicated.

Any allowance in the sample calculation for losses to follow-up should also be reported.

Item 8a: Sequence generation

Method used to generate the random allocation sequence.

No change from standard CONSORT item.

Example 1—“After a 4-week placebo run-in, eligible patients were randomly assigned, according to a computer generated allocation schedule, to 1 of 2 treatment sequences: montelukast and placebo-matching salmeterol or salmeterol and placebo-matching montelukast. After a 2-week washout, patients crossed over to the other treatment.”43

Example 2—“Eligible subjects were randomized in a 1:1 allocation to one of two treatment sequences—denosumab/alendronate or alendronate/denosumab—and received each treatment for 1 year.”44

Explanation—In crossover RCTs, allocation sequence refers to the order in which interventions are received. The allocation might be to sequence one, in which participants have A followed by B, or to sequence two, in which participants have B followed by A.

Item 10: Implementation

Who generated the random allocation sequence, who enrolled participants, and who assigned participants to the sequence of interventions.

Standard CONSORT item—Who generated the random allocation sequence, who enrolled participants, and who assigned participants to interventions.

Explanation—For this item, “the sequence of” was included before interventions as participants are randomised to a sequence of interventions rather than one intervention.

Item 12a: Statistical methods

Statistical methods used to compare groups for primary and secondary outcomes which are appropriate for crossover design (that is, based on within participant comparison).

Standard CONSORT item—Statistical methods used to compare groups for primary and secondary outcomes.

Example 1—“Cross-over analyses for health related quality of life scores averaged the between-treatment difference for each patient within each sequence and then across both sequences, providing an estimate of treatment effect. The estimated treatment difference, 95% CI and P value were adjusted for period and sequence effects in the analysis of variance model” (emphasis added).39

Example 2—“A generalized linear mixed models approach was used to estimate differences between periods of electrical stimulation and no stimulation while accounting for within-subject correlations arising from the crossover design” (emphasis added).45

Example 3—“Statistical analysis allowed for the comparison of both treatment groups with respect to baseline information and subsequent comparison at 2 and 4 weeks for treatment effect. The investigator’s assessment and patient’s assessment of treatment were analysed using Gart’s test for binary responses, which takes treatment order [strictly period] into account” (emphasis added).46

Example 4—“Side effects and patient preferences were analyzed descriptively and using McNemar’s test” (emphasis added).47

Example 5—“Prescott’s test was used to analyze the primary end point to test the significance of difference between the two treatments in the presence of period effects” (emphasis added).39

Explanation—In line with recommendations made by the International Committee for Medical Journal Editors and the CONSORT group, analytical methods should be described “with enough detail to enable a knowledgeable reader with access to the original data to verify the reported results” (http://www.icmje.org/recommendations/browse/manuscript-preparation/preparing-for-submission.html#d). Identification of the crossover design and the statistical methods used allows readers to evaluate the methods of analysis.

The analysis of a crossover trial should respect the within participant nature of the comparisons. The Methods section should specify which method of analysis was used. This should clearly show how the within participant analysis has been constructed, for example using t tests on within participant differences, or analysis of variance with participant, period, and treatment effects. If period and carry over effects have been modelled, then this should be reported. Similarly, for a binary outcome, conditional logistic regression provides an alternative way of conducting the Mainland-Gart test. The consequences of an analysis not accounting for a within participant comparison could overestimate the variance for the treatment effect.

In some crossover trials participants are measured on the outcome variable at the beginning and at the end of both periods, and the treatment effect is estimated using the change score from each period. This intuitive approach is claimed to eliminate carry over effect; however it could produce a less precise and even biased estimate of treatment effect,4849 and therefore should be discouraged.

While missing data raise the same generic issues in crossover trials as in other designs, the specifics are more complicated. The analysis model, in the absence of missing data, should be identified and the role of baseline data needs to be carefully considered because often baseline adjustment increases the standard error. A mixed model of all available data (eg, in this context, with a mixture of fixed and random effects) is typically the preferred first step, with the contextually appropriate adjustment for within subject dependence, and is valid under Rubin’s “missing at random” assumption. Broadly, this states that the distribution of later outcome data, given treatment sequence and earlier data, is the same whether or not those data are observed. Analysis of the complete records gives a valid intention to treat estimate by assuming that the distribution of the outcomes given baseline and treatment sequence is the same, whether or not they are observed (that is, missing at random). We can explore the robustness of the conclusion to this untestable assumption by multiply imputing the data and forcing the distribution of imputed outcomes to differ from the observed ones given baseline and treatment sequence. The use of multiple imputation, imputing from subsets of patients (rather than single mean imputation, last value carried forward, or best/worst imputation) is welcome because the imputed data are contextually plausible and appropriately reflect the variability.50

Results

Item 13a: Participant flow

The numbers of participants who were randomly assigned, received intended treatment, and were analysed for the primary outcome, separately for each sequence and period (a flow diagram is strongly recommended; see fig 1).

Fig 1
Fig 1

CONSORT flow diagram for crossover trials (would need modification if more than two groups and/or periods). A=intervention; B=control or other intervention; CONSORT=Consolidated Standards of Reporting Trials. In many cases a washout period can be shown

Standard CONSORT item—For each group, the numbers of participants who were randomly assigned, received intended treatment, and were analysed for the primary outcome.

Example 1—See figure 2 (adapted from Chen et al51).

Fig 2
Fig 2

Example of flow diagram. NCEP=National Cholesterol Education Program

Example 2—See figure 3 (adapted from Marchetti et al52).

Explanation—The flow diagram is a key element of the CONSORT statement and has been widely adopted. For crossover trials it is important to understand the flow of participants across periods. Although we recommend a flow diagram for communicating the flow of participants throughout the study, the exact form and content can vary in relation to the specific features of a trial. We recommend using vertical alignment and including a timescale.

Item 13b: Losses and exclusions

Number of participants excluded at each stage, with reasons, separately for each sequence and period.

Standard CONSORT item—For each group, losses and exclusions after randomisation, together with reasons.

Example 1—“One subject assigned to receive active placebo first withdrew because of a scheduling conflict before taking any study medication. Two subjects assigned to receive pregabalin first withdrew in the first period because of adverse events. The remaining 26 subjects completed the study.”53

Example 2—“Of the 23 patients who provided consent, 17 were randomized to a treatment sequence (9 to pancrelipase then placebo, 8 to placebo then pancrelipase). Sixteen patients completed the study; 1 patient (pancrelipase/placebo sequence) withdrew consent on day 2 of the first treatment period.”54

Explanation—Participants who drop out part way through the trial will have their outcome assessed for only one intervention. Dropping out might be informative; for example, they could be dissatisfied with the treatment they were given and so do not wish to try any other treatments. This could bias the results.

Authors should indicate the loss of participants for each intervention, separately for each sequence and period, possibly within the flow diagram with reasons if possible.

There are statistical methods to deal with incomplete data (see Item 12a).

Item 15: Baseline data

A table showing baseline demographic and clinical characteristics by sequence and period.

Standard CONSORT item—A table showing baseline demographic and clinical characteristics for each group.

Example 1—See table 3 (adapted from Fogel et al43).

Table 3

Baseline demographic and clinical characteristics by sequence only

View this table:

Example 2—See table 4 (adapted from Valentino et al55).

Table 4

Baseline demographic and clinical characteristics by sequence and by total

View this table:

Explanation—Random assignment by individual ensures that any differences in group characteristics at baseline are the result of chance rather than some systematic bias.2 For randomised crossover trials, it is desirable to know whether baseline characteristics that can be affected by the intervention have returned to their initial state at the beginning of the second period. The by sequence information is needed to assess whether randomisation has achieved balance between the sequences for important variables at the start of the trial. The by period information is helpful for readers to understand whether the treatment effect in the next period is confounded by the changing participant characteristics between periods. Characteristics that remain the same at the start of the two periods, such as sex and age, can be presented once; however, unstable prognostic factors and baseline value of the main outcome must be checked at the beginning of each period. If the characteristic can change over time, then a baseline table by sequence only precludes inference of differences between period (that is, treatment).

Item 16: Numbers analysed

Number of participants (denominator) included in each analysis and whether the analysis was by original assigned groups.

Standard CONSORT item—For each group, number of participants (denominator) included in each analysis and whether the analysis was by original assigned groups.

Explanation—The number of participants who contribute to the analysis of a trial is essential to interpreting the results. The analysis of crossover trials has to account for the paired nature of the design; the numbers analysed for each outcome should be equal to the numbers of within participant differences or contrasts that were possible. However, not all participants might contribute to the analysis of each outcome. In a crossover trial, when participants do not contribute to the analysis from one period, the corresponding period may be lost. Assuming no carry over or period effect, if imputation is undertaken the data could be salvaged and when no imputation is undertaken the data are lost, and this becomes a power issue. As the sample size and hence the power of the study is calculated on the assumption that all participants will provide information, the number of participants contributing to a particular analysis should be reported so that any potential drop in statistical power can be assessed. When there is carry over or a period effect, missing data will result in a biased estimate. In addition, and as explained in detail in the CONSORT 2010 guideline,2 it should be specified whether a per protocol or an intention to treat analysis was followed.

Item 17a: Outcomes and estimation

For each primary and secondary outcome, results including estimated effect size and its precision (such as 95% confidence interval) should be based on within participant comparisons. In addition, results for each intervention in each period are recommended.

Standard CONSORT item—For each primary and secondary outcome, results for each group, and estimated effect size and its precision (such as 95% confidence interval).

Example 1—See table 5 (adapted from Graff et al54).

Table 5

Coefficient of fat absorption results by treatment and severity of exocrine pancreatic insufficiency

View this table:

Example 2—See table 6 (adapted from Rubio-Aurioles et al38).

Table 6

Treatment comparisons and changes between baseline and treatment endpoint for secondary outcomes

View this table:

Example 3—“Eighty patients (70%) preferred pazopanib; the most common reasons included better overall quality of life (QoL) and less fatigue. Twenty-five patients (22%) preferred sunitinib; the most common reasons included less diarrhoea and better overall QoL. Physician preferences were consistent with patient preferences. More physicians preferred to continue their patients on pazopanib (61%) than on sunitinib (22%), with 17% stating no preference.”39

Example 4—See table 7 (adapted from O’Connor et al56).

Table 7

Incidence rate ratios (IRRs) with 95% confidence intervals for effects of treatment, time, and treatment–time interaction on behaviour and affect scores after taking account of age, gender, and dementia severity

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Explanation—When reporting the results of randomised crossover trials, point estimates with confidence intervals should be reported for primary and secondary outcomes; this is the same as the standard CONSORT guideline except that these results should be based on the appropriate within participant analysis. Results should not be presented as though they are from a parallel group trial or by double counting the participants. Ideally, as the correlation impacts on the power of the study, the correlation coefficient for each primary outcome being analysed should also be provided to help with the planning of future crossover trials.

For binary outcomes a presentation using a matched tabulation format is desirable because it allows the reader to see the concordant and discordant pairs. The matched tabulation facilitates the use of such trials in future meta-analyses because it allows appropriate formulas to be used to adjust the between treatment variance downwards by accounting for the within participant correlation, even when not available.575859 Presentation of the 2×2 table of results from a crossover design in a parallel trial format does not allow for appropriate adjustments of the between treatment variance.57 The paired presentation is also helpful for future sample size calculations. However, in many circumstances the data will be analysed by a model that accounts for the design and is displayed as shown in example 4.

Presentation of the results for each intervention in each period is recommended because these can be used to help understand any treatment by period interaction, regardless of how the trial investigators handled it in their analysis (see table 7 of Li et al30).

Ideally, participant preference outcomes should also be reported at the participant level. For example, the participants should be split according to those who prefer intervention A and those who prefer intervention B, and analysed using McNemar’s test or, if allowing for period, the Mainland-Gart test or Prescott’s test.

Item 19: Harms

Describe all important harms or unintended effects in a way that accounts for the design (for specific guidance see CONSORT for harms32).

Standard CONSORT item—All important harms or unintended effects in each group (for specific guidance see CONSORT for harms32).

Example—See table 8 (this example is fictional).

Table 8

Adverse events

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Explanation—The types of adverse events and the overall frequency under each intervention should be described. In addition, for crossover trials, presenting concordant and discordant pairs of adverse events or providing estimates of effect and precision (when between group comparisons were made) will inform the relative safety of the interventions tested. The table provides an example of how to tabulate adverse events.

Discussion

Item 20: Limitations

Trial limitations, addressing sources of potential bias, imprecision, and, if relevant, multiplicity of analyses. Consider potential carry over effects.

Standard CONSORT item—Trial limitations, addressing sources of potential bias, imprecision, and, if relevant, multiplicity of analyses.

Example 1—“The 24-hour washout period may have been insufficient to eliminate the effects of stimulation. Potential carryover effects should be addressed by the use of alternative study designs (eg, parallel groups, longer study/washout periods, stepped-wedge designs).”45

Example 2—“Strengths of this study include blinding of study treatments and a cross-over design, where patients were exposed to both treatments in similar health states. This allowed for detection of differences in tolerability not confounded by differences in health states and for each patient to act as their own control. In addition, the 2-week washout period and random assignment minimized possible effects of the order of treatment and carryover.”39

Example 3—“Finally, it is possible that the crossover design could have obscured differences in the period on and off HCQ [hydroxychloroquine]. While allowing for a washout period may have helped rule out such a possibility, the pilot study suggested no such washout period was required.”60

Explanation—A limitation with the crossover design is that the treatment from the first period might affect the results from the second period, either to improve the outcome with the opposite treatment or to suppress the effect. This carry over effect could potentially render a crossover trial invalid and reporting of such a limitation is unlikely to be found given that it would invalidate the trial results. Possible limitations that should be reported include losses to follow-up before the second intervention is applied, and mixing up the interventions so that the sequence applied was not the one to which the participant was randomised. The appropriateness of a crossover design in terms of the stability of the disease over the duration of the trial could also be discussed.

More complicated trial designs

In the previous sections we discussed reporting of the simple 2×2 trial design where each participant is randomised to one of two sequences in which to receive the two competing interventions. More complicated variations of the crossover design include comparing three or more interventions (please see the CONSORT extension for multiarm trials61) and cluster crossover randomised trials. In a cluster crossover RCT, each cluster receives multiple interventions in a randomised sequence.62 A recent review found that there is a need to ensure an appropriate analysis is undertaken and reporting needs to be improved.63 The development of an extension of CONSORT to cluster crossover trials is underway (Joanne McKenzie, personal communication).

There could also be issues of repeated measurements (that is, measurements taken at several time points) or multiplicity within participants in crossover trials (for example, both eyes are assessed within participants). Other, less frequently used versions of the crossover design include bioequivalence studies, Balaam’s design, extra period designs, n-of-1 designs, and an incomplete block design.17

Comment

Reports of RCTs should include key information on the methods and findings to allow readers to accurately interpret the results. This information is particularly important for meta-analysts attempting to extract data from such reports. The CONSORT 2010 statement provides the latest recommendations from the CONSORT group on essential items to be included in the report of a RCT. In this paper we introduce and explain corresponding updates in an extension of the CONSORT checklist specific to reporting randomised crossover trials.

Use of the CONSORT statement for the reporting of two group parallel trials is associated with improved reporting quality.64 We believe that the routine use of this proposed extension to the CONSORT statement will eventually result in improvements to crossover designs. When reporting a randomised crossover trial, authors should address all 25 items on the CONSORT checklist by using this document in conjunction with the main CONSORT guidelines.3 Authors might also find it useful to consult the CONSORT extensions for other trial designs (available at www.consort-statement.org/extensions).

The CONSORT statement can help researchers to design trials in the future and can guide peer reviewers and editors in their evaluation of manuscripts. Many journals recommend authors adhere to the CONSORT recommendations in their instructions to authors. We encourage them to direct authors to this and to other extensions of CONSORT for specific trial designs. The most up to date versions of all CONSORT recommendations can be found online (www.consort-statement.org).

Acknowledgments

We thank James Carpenter and Mike Kenward for their input into how missing data are handled in crossover trials. We also thank Sally Hopewell, Nikolaos Pandis, and Drummond Rennie, and other members of the CONSORT Executive for helpful comments, as well as the peer reviewers Stephen Senn, Francois Curtin, and Karla Hemming. Note that small parts of the text in this manuscript are necessarily similar to other CONSORT articles.

Footnotes

  • Contributors: DGA and DE initiated the work. KD, TL, DE, and DGA drafted the manuscript and all authors reviewed it. KD, TL, and DE approved the final version (DGA died in June 2018). KD is the guarantor. The corresponding author attests that all listed authors meet authorship criteria and that no others meeting the criteria have been omitted.

  • Funding: This study received no specific funding.

  • Competing interests: All authors have completed the ICMJE uniform disclosure form at www.icmje.org/coi_disclosure.pdf and declare: TL reports grants from National Eye Institute, National Institutes of Health, and grants from National Library of Medicine, National Institutes of Health during the conduct of the study.

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

References