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Perspectives in diabetes
Correlation study of adenosine deaminase and its isoenzymes in type 2 diabetes mellitus
  1. Lokendra Bahadur Sapkota1,
  2. Sangita Thapa2,
  3. Nuwadatta Subedi3
  1. 1Department of Biochemistry, Chitwan Medical College, Bharatpur, Nepal
  2. 2Department of Microbiology and Immunology, Chitwan Medical College, Bharatpur, Nepal
  3. 3Department of Forensic Medicine and Toxicology, Gandaki Medical College, Pokhara, Nepal
  1. Correspondence to Dr Lokendra Bahadur Sapkota; sdrloken{at}gmail.com

Abstract

Objective Adenosine deaminase (ADA) plays an important role in cell-mediated immunity and modulation of insulin activity. Its clinical and diagnostic significance in Nepalese type 2 diabetes is not yet characterized. So, this study's objective was to determine the isoenzymatic activities of ADA (ADA1, ADA2, and total ADA) and show its correlation with demographic, anthropometric, and biochemical characteristics of type 2 Nepalese subjects with diabetes.

Research design and methods This is a hospital-based cross-sectional study including 80 type 2 diabetes mellitus (DM) patients and same number of age-matched and sex-matched healthy controls. Data were collected using preformed set of questionnaires and biochemical data were obtained from the laboratory analysis of the patient's blood samples. Statistical analysis was performed with SPSS V.20.

Results A significantly higher (p<0.001) mean values of body mass index (BMI), fasting blood sugar (FBS), postprandial blood sugar (PPBS), glycated hemoglobin (HbA1c), and lipid profiles except high-density lipoprotein cholesterol (HDL-C) were found in type 2 diabetic cases compared with controls. Serum ADA activities were significantly higher in cases compared with controls (p<0.001) showing significant positive correlation (p<0.05) with FBS, PPBS, HbA1c, and alcoholism; while no correlation was found with age, sex, ethnicity, BMI, waist–hip ratio, dietary habits, smoking, and duration of diabetes.

Conclusions Serum ADA activities were significantly higher in type 2 diabetic patients compared with controls having significant positive correlation with glycemic parameters. Serum ADA and its isoenzymes could be used as biomarkers for assessing glycemic status in patients with type 2 DM.

  • Adenosine
  • Type 2 Diabetes

This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/

https://doi.org/10.1136/bmjdrc-2016-000357

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    Footnotes

    • Contributors This work was carried out in collaboration between all the authors. LBS prepared the questionnaire, collected the samples, and performed biochemical tests. LBS, ST, and NS analyzed the results, prepared, and refined the manuscript.

    • Funding This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors.

    • Competing interests None declared.

    • Ethics approval Institutional ethical committe, Manipal.

    • Provenance and peer review Not commissioned; externally peer reviewed.

    • Data sharing statement No additional data are available.